Reverse Complement

🧬 Reverse Complement

Reverse-complement DNA/RNA sequences directly in your browser. Perfect for primers and probes.

Supports FASTA, FASTQ, SAM, and line-by-line raw sequences. Reverses qual scores and handles IUPAC codes!

Input Sequences:

Processing Mode: By Line - Reverse complement each line independently (for Excel columns) Autodetect - Auto-detect format (FASTA, FASTQ, or SAM) and process accordingly

Output:

Examples:

Simple Sequence:

Input: ATCGATCG

Output: CGATCGAT

FASTA Format (Autodetect mode):

>primer1
ATCGATCG
>primer2
GCTAGCTA

Outputs reverse complements with headers preserved.

Excel Column (By Line mode):

ATCGATCG
GCTAGCTA
TTAACCGG

Each line is reverse-complemented independently.

IUPAC Ambiguity Codes:

Input: RYWSMKBDHVN

Output: NBDHVKMWSRY

R↔Y, W↔W, S↔S, M↔K, B↔V, D↔H, N↔N

FASTQ Format (Autodetect mode):

@read1
ATCGATCG
+
IIIIIIII

Reverse complements sequence AND quality scores!

SAM Format (Autodetect mode):

Paste SAM alignment lines - sequence (field 10) and quality (field 11) will be reverse complemented. Headers preserved.

Note: This tool runs entirely in your browser using JavaScript. Your sequences never leave your computer!